![]() I know it belongs in the non linear category (i think) but its not in the drop down menu of available analysis. Up to now i was using linear regression for getting the values, but according to the manufacturer this is not the correct way to process the data. The data type are simple and straight forward, meaning i get some optical density values from samples of known concentration and from that i interpolate to find the values of samples of unknown concentration (cytokines). I am using GraphPad Prism 4 for the statistics and i cant find anywhere this type of analysis - it simply isnt there. I am doing research ELISA (I am a medical doctor) and the kit manufacturer says on the manual that i should do 4 parameter logistic analysis of the results i get. ![]() Dont know mutch about statistics and i thought some of you might be able to help.
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